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human αcgrp  (Tocris)


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    Structured Review

    Tocris human αcgrp
    Human αcgrp, supplied by Tocris, used in various techniques. Bioz Stars score: 92/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+%CE%B1cgrp/alpha-CGRP+(human)/pmc09207571-83-8-10
    Average 92 stars, based on 7 article reviews
    human αcgrp - by Bioz Stars, 2026-09
    92/100 stars

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    Related Articles

    Incubation:

    Article Title: Low-dose interleukin-2 reverses chronic migraine-related sensitizations through peripheral interleukin-10 and transforming growth factor beta-1 signaling.
    Article Snippet: After a 2–3 min baseline measurement in Tyrode’s solution, neurons were perfused with 50 nM PACAP1-38 (Tocris, Minneapolis, MN, 1136) for 1 min followed by washing with Tyrode’s for 4 min. .. Subsequently, the coverslip was incubated with 3 μM human αCGRP (Tocris, 3012) for 1 min followed by washing with Tyrode’s for 4 min. CGRP and PACAP were freshly diluted from the stock (aliquots at − 80 ◦C) in Tyrode’s solution before each experiment. ..

    Article Title: Low-dose interleukin-2 reverses chronic migraine-related sensitizations through peripheral interleukin-10 and transforming growth factor beta-1 signaling
    Article Snippet: After a 2–3 min baseline measurement in Tyrode’s solution, neurons were perfused with 50 nM PACAP 1-38 (Tocris, Minneapolis, MN, 1136) for 1 min followed by washing with Tyrode’s for 4 min. .. Subsequently, the coverslip was incubated with 3 μM human αCGRP (Tocris, 3012) for 1 min followed by washing with Tyrode’s for 4 min. CGRP and PACAP were freshly diluted from the stock (aliquots at −80 °C) in Tyrode’s solution before each experiment. ..

    Cell Culture:

    Article Title: Paracrine signalling by cardiac calcitonin controls atrial fibrogenesis and arrhythmia.
    Article Snippet: For the experiments with TGFβ1 and CT stimulation, we used commercially available donors of human primary atrial fibroblasts (CC-2903, Lonza), which were maintained and cultured in the same medium as outlined above. .. Experiments were carried out at cell passages P3–P4 and cells were cultured in serum-free medium for ~16 h before intervention and treatment with 100 or 500 nM of human CT (H-2250, Bachem), 10 or 100 nM of human αCGRP (3012, Tocris Bioscience), 10 nM of BIBN4096 (4561,Tocris Bioscience), 100 nM of sCT8-32 (4037182, Bachem,) or 10 ng/ml of TGFβ1 (HZ-1011, Proteintech). ..



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    a Sequence alignment of the 37mer signaling peptide, CGRP. Conserved residues represented by *. Red box indicates the targeting peptide sequence (B cell epitope) of the human <t>αCGRP</t> peptide immunogen, p4796kb, and the <t>rat</t> <t>αCGRP</t> peptide immunogen, p5830kb. Anti-CGRP antibody titers over time in rat ( n = 5) ( b ), guinea pig ( n = 3) ( c ), and NHP ( n = 3) ( d ). Anti-human αCGRP (K35K) ( e ) and anti-rat αCGRP (K35E) ( f ) IgG titers in sera collected post-immunization in rats injected with p4796kb or p5380kb ( n = 5 per group). g Serum concentrations (μg/mL) of anti-CGRP antibodies. Red arrowheads indicate dosing time points. Individual data points are presented on each graph.
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    a Sequence alignment of the 37mer signaling peptide, CGRP. Conserved residues represented by *. Red box indicates the targeting peptide sequence (B cell epitope) of the human <t>αCGRP</t> peptide immunogen, p4796kb, and the <t>rat</t> <t>αCGRP</t> peptide immunogen, p5830kb. Anti-CGRP antibody titers over time in rat ( n = 5) ( b ), guinea pig ( n = 3) ( c ), and NHP ( n = 3) ( d ). Anti-human αCGRP (K35K) ( e ) and anti-rat αCGRP (K35E) ( f ) IgG titers in sera collected post-immunization in rats injected with p4796kb or p5380kb ( n = 5 per group). g Serum concentrations (μg/mL) of anti-CGRP antibodies. Red arrowheads indicate dosing time points. Individual data points are presented on each graph.
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    Image Search Results


    a Sequence alignment of the 37mer signaling peptide, CGRP. Conserved residues represented by *. Red box indicates the targeting peptide sequence (B cell epitope) of the human αCGRP peptide immunogen, p4796kb, and the rat αCGRP peptide immunogen, p5830kb. Anti-CGRP antibody titers over time in rat ( n = 5) ( b ), guinea pig ( n = 3) ( c ), and NHP ( n = 3) ( d ). Anti-human αCGRP (K35K) ( e ) and anti-rat αCGRP (K35E) ( f ) IgG titers in sera collected post-immunization in rats injected with p4796kb or p5380kb ( n = 5 per group). g Serum concentrations (μg/mL) of anti-CGRP antibodies. Red arrowheads indicate dosing time points. Individual data points are presented on each graph.

    Journal: Communications Medicine

    Article Title: Preclinical characterization of an active immunotherapy targeting calcitonin gene-related peptide

    doi: 10.1038/s43856-025-00870-2

    Figure Lengend Snippet: a Sequence alignment of the 37mer signaling peptide, CGRP. Conserved residues represented by *. Red box indicates the targeting peptide sequence (B cell epitope) of the human αCGRP peptide immunogen, p4796kb, and the rat αCGRP peptide immunogen, p5830kb. Anti-CGRP antibody titers over time in rat ( n = 5) ( b ), guinea pig ( n = 3) ( c ), and NHP ( n = 3) ( d ). Anti-human αCGRP (K35K) ( e ) and anti-rat αCGRP (K35E) ( f ) IgG titers in sera collected post-immunization in rats injected with p4796kb or p5380kb ( n = 5 per group). g Serum concentrations (μg/mL) of anti-CGRP antibodies. Red arrowheads indicate dosing time points. Individual data points are presented on each graph.

    Article Snippet: Microtiter 96-well plates were 1st coated with 100 μL of 2 μg/mL of the full-length synthetic human or rat αCGRP 1-37 (Bachem, CA, product number 4013281) peptide at room temperature (RT) for 1 h and 4 °C overnight.

    Techniques: Sequencing, Injection